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5–four μmol/L variety,[156][157] the focus of serotonin in synaptic vesicles was calculated at 270 mM.[182] next vesicular launch, the ensuing focus of serotonin while in the synaptic cleft, to which serotonin receptors are exposed, is estimated for being about three hundred μmol/L. Therefore, whilst in vitro receptor binding affinities, effi
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